Human Nesfatin 1 (NES1) ELISA Kit

Cat.#:

JL15348

Regulatory Status:

RUO

Alternative:

Nesfatin 1

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.78-50ng/mL

Sensitivity:

0.33ng/mL

Specificity:

This kit recognizes Human NES1 in samples. No significant cross-reactivity or interference between Human NES1 and analogues was observed.

Sample Type:

Serum;plasma and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

Email *

Message *

Please Sign In.

Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| NES1

Nesfatin-1 is a neuropeptide produced in the hypothalamus of mammals. It participates in the regulation of hunger and fat storage.Increased nesfatin-1 in the hypothalamus contributes to diminished hunger, a 'sense of fullness', and a potential loss of body fat and weight.Nesfatin-1 is a polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin-2 (NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues.Nesfatin-1 is expressed in the hypothalamus, in other areas of the brain, and in pancreatic islets, gastric endocrine cells and adipocytes.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human NES1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	97-108	105

| Linearity

Samples were spiked with high concentrations of Human NES1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-104	87-108	105-115
1 : 4	Average (%)	93	98	110